Analysis Note

ControlHuman Lymphocytes

Application

Milli-Mark Anti-CD4 -FITC Antibody, clone MT310 is an antibody against CD4 for use in FC.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD4 is a 55 kDa glycoprotein with four immunoglobulin-like external domains, a transmembrane domain and a highly conserved intracellular domain (Leong, 1999). CD4 acts as a coreceptor in MHC class II-restricted antigen-induced T-cell activation. Further, CD4 has a role in the regulation of T-B lymphocyte adhesion in the absence of antigen recognition, and in thymic differentiation (Piatier-Tonneau, 2002). CD4 is expressed on thymocyte subsets, it marks helper/inducer T cells, and it is expressed in 55-65% of mature peripheral T cells. Subpopulations of suppressor or cytotoxic T cells can be identified among CD4-positive T cells. CD4 is also expressed on monocytes/macrophages, Langerhans cells and other dendritic cells. CD4 is not expressed on B cells. Anti-CD4, MT310, was included in the Second International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with the CD4 antigen (Miedema, 1986).

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 kappa conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Quality

Evaluated by flow cytometry using human lymphocytes.

Specificity

Antibody recognizes Human CD4 antigen.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

55 kDa (Leong, 1999)